personal_ws-1.1 en 67 aequeous agarose annexin autoactivation billman biorad biosystems bmdms calmodulin capsulatum caspase caspases cdna chromobacterium copc coverslips dimerization dimerizer dmrb dt effectors gasdermin gasdermins genotyping gggsdma gggsmde granuloma granulomas gsdm gsdmd gsdmes histoplasma hoechst hsgsdmd igg immunofluorescence invitrogen lysed lysine mowiol mycoplasma neomycin oligo optimem overexpression plasmid plasmids primerblast proteasome qpcr quantstudio rnas rneasy supermix supernatant tagless timepoint titrate titration transcriptase transfected transfection trizol ug violaceum vitro zach