personal_ws-1.1 en 103 agarose annexin aspergillus autoactivation autophagy billman biorad biosystems bmdms calmodulin capsulatum caspase caspases cdna chromobacterium cleavable coa coli copc coverslip coverslips dimerization dimerizer dmrb dt effectors elute epigenetic etoh fluorophore fumarate gasdermin gasdermins genotyping gggsdma gggsmde granuloma granulomas gsdm gsdmd gsdmes histoplasma hoechst hsgsdmd igg immunofluorescence inflammasome invitrogen kda lentivirus lysed lysine lysing lysis macrophage methyltransferases microcentrifuge monocyte monocytes mowiol mtor mycoplasma myeloid neomycin neutrophil ng nigericin nystain oligo optimem overexpression petri plasmid plasmids primerblast protease proteasome pyroptosis qpcr quantstudio resuspended rnas rneasy sera streptavidin successful supermix supernatant supernatants tagless timepoint titrate titration transcriptase transfect transfected transfection trizol ug uncleavable violaceum vitro zach