personal_ws-1.1 en 75 agarose annexin aspergillus autoactivation billman biorad biosystems bmdms calmodulin capsulatum caspase caspases cdna chromobacterium copc coverslips dimerization dimerizer dmrb dt effectors etoh gasdermin gasdermins genotyping gggsdma gggsmde granuloma granulomas gsdm gsdmd gsdmes histoplasma hoechst hsgsdmd igg immunofluorescence invitrogen lentivirus lysed lysine lysis macrophage mowiol mycoplasma neomycin nystain oligo optimem overexpression petri plasmid plasmids primerblast proteasome qpcr quantstudio resuspended rnas rneasy successful supermix supernatant tagless timepoint titrate titration transcriptase transfected transfection trizol ug violaceum vitro zach